Microboilogy Essay Example | Topics and Well Written Essays - 1250 words

Microboilogy - Essay Example 5. a.) Melted agar should be cooled to 45-47 degree Celsius before pouring to the agar plate since pouring it in a higher temperature might kill the organisms and thus falsely decrease the microbial count. c.) Aseptic technique should always be employed in order to prevent other microorganism from contaminating the set-up. Any contamination would lead to difficulty, if not, production of erroneous microbial count. 6.) Used media may contain harmful and infectious organisms; moreover it should always be treated as biologically hazardous waste. Required waste disposal can be costly; nevertheless used media can never be disposed directly into the waste basket. To date, autoclaving serves as a cheap but effective way to sterilized waste materials in microbiology laboratory. The fact that most microorganisms can not withstand autoclaving, this simple technique would conveniently address the issue on the proper disposal of used media. An autoclave must be available for the laboratory and must be operated by properly trained personnel. On a regular basis, biological indicators or spore strips should be included in autoclave loads as to verify its functionality and efficiency of sterilization. Temperature-sensitive tape, thermograph, or other biological indicators should also be used to monitor each autoclave. Scientist have investivated microbial behavior in area such as Clostridium botulinum in modified atmospheric processing, Escherichia coli in apple processing, Salmonella and E. coli in vegetable seed sprouting etc. Some studies shows that bacteria converts food